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Cdc27 Rabbit Monoclonal Antibody
Catalog #£ºEAB21838
  SKU-Pack Size Availability Size Price
EAB21838-100UL In Stock 100ul €297.70
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SBT BioChem Srl Phone£º+39 05 7156 8147
Via Ponte di Bagnolo, 811 Bologna, E-mail£ºinfo@sbtbiochem.it
Italy Italy Web£ºwww.sbtbiochem.it
Product Information
Applications WB, IHC-P, IF/ICC, FC
Species Reactivity Human, Rat
Host / Isotype Rabbit IgG
Clonality Monoclonal
Applications Dilutions WB=1:500-2000¡¡
IHC-P=1:100-500
IF/ICC=1:50-200
FC=1:10-100
MW (kDa) 92
Conjugate Unconjugate
Specificity Cdc27 Rabbit Monoclonal Antibody detects endogenous levels of Cdc27 protein.
Purification Affinity purification
Concentration 1mg/ml
Format Liquid
Formulation In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping Gel Pack
Storage Store at -20¡ãC least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4¡ãC for 1-2 weeks.
Research Use For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry

Reactivity Key

H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s) Anaphase Promoting Complex 3; Cell division cycle 27; ANAPC3; APC3; CDC27; CDC27Hs; H-NUC; HNUC; NUC2
Gene Aliases CDC27
UniProt ID

P30260 

Entrez Gene ID

996 

Product Description

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. 

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Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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