Polyclonal Antibodies - AMPKα1/2 Rabbit Polyclonal Antibody

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AMPKα1/2 Rabbit Polyclonal Antibody

Catalog #：EAB10168

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10168-30UL	In Stock	30ul	€115.70
EAB10168-100UL	In Stock	100ul	€258.70
EAB10168-200UL	In Stock	200ul	€440.70

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email us at info@ebiocell.com

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	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat, Monkey, Fish
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	64
Conjugate	Unconjugate
Specificity	AMPKα1/2 Rabbit Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	AMPK; AMPK1; AMPKa1; AMPKalpha1; AMPKalpha1/AMPKalpha2; PRKAA1/PRKAA2; PRKAA1/2; AMPKα; AMPKα1/2; AMPKα1/AMPKα2;
Gene Aliases	PRKAA1/PRKAA2
UniProt ID	Q13131, P54646
Entrez Gene ID	5562, 5563

Product Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Image Gallery

Western blot analysis of extracts on different cells, using AMPKα1/2 Rabbit Polyclonal Antibody (EAB10168) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: K562
Lane2: HL60
Lane3: Mouse lung

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue, using AMPKα1/2 Rabbit Polyclonal Antibody (EAB10168) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol