Monoclonal Antibodies - eIF4A1 Mouse Monoclonal Antibody

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eIF4A1 Mouse Monoclonal Antibody

Catalog #：EAB14487

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB14487-30UL	In Stock	30ul	€115.70
EAB14487-100UL	In Stock	100ul	€258.70
EAB14487-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	48
Conjugate	Unconjugate
Specificity	eIF4A1 Mouse Monoclonal Antibody detects endogenous levels of eIF4A1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	BM-010; DDX2A; EIF-4A; eIF-4A-I; Eif4; EIF4A; eIF4A-I; Eukaryotic initiation factor 4A-I; EIF4A1
Gene Aliases	EIF4A1
UniProt ID	P60842
Entrez Gene ID	1973

Product Description

The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA – this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.

Product Image Gallery

Western blot analysis of extracts on different cells, using eIF4A1 Mouse Monoclonal Antibody (EAB14487) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: Hela
Lane2: 293T
Lane3: HepG2

Immunohistochemical analysis of paraffin-embedded Human lung caricnoma tissue, using eIF4A1 Mouse Monoclonal Antibody (EAB14487) at 1:200 dilution.

Immunofluorescence analysis of Hela cell, using eIF4A1 Mouse Monoclonal Antibody (EAB14487) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol