The original formulation of McCoy’s 5A Medium fulfills Novikoof's needs of culturing kidney cancer cells and supports the propagation of Walker 256 cancer cells as well as various human and rats’ normal and transformed cells. Iwakata and Grace were the first two to modify this medium. This product is formulated with higher inositol and glucose concentrations. This medium also contains L-glutamine.
The product is prepared using water-for-injection.

Product Specifications:
With (+)
• 3.0 g/L D-Glucose
• L-Glutamine
• Bacto Peptone
Without (-)
• sodium pyruvate
• HEPES