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Puromycin dihydrochloride (CAS: 58-58-2)
Catalog #£ºEBC51156
  SKU-Pack Size Availability Size Price
EBC51156-1ML In Stock 1mL(10mM in DMSO) €76.70
EBC51156-25MG In Stock 25mg €102.70
EBC51156-50MG In Stock 50mg €180.70
EBC51156-100MG In Stock 100mg €310.70
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Product Information
Synonym(s) Puromycin HCl, Stylomycin hydrochloride, CL13900, P-638
Chemical Name (2S)-2-Amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide dihydrochloride
Application Puromycin dihydrochloride is an analog of aminoacyl-tRNA
CAS Number 58-58-2
Purity ¡Ý99.0%
Molecular Weight 544.43
Molecular Formula C₂₂H₃₁Cl₂N₇O₅
SMILES CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl
Target & IC50 DNA synthesis
Solubility DMSO: 54.4 mg/mL (100 mM)
Water: 92 mg/mL (169 mM)
Preparing Stock Solutions
ConcentrationSolventMass 1 mg 5 mg 10 mg
1 mM 1.8368 ml 9.1839 ml 18.3678 ml
5 mM 0.3674 ml 1.8368 ml 3.6736 ml
10 mM 0.1837 ml 0.9184 ml 1.8368 ml
50 mM 0.0367 ml 0.1837 ml 0.3674 ml
Shipping Gel Pack
Storage Store at -20¡ãC
Research Use For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.
Product Description

Puromycin dihydrochloride, also known as Stylomycin hydrochloride, is an aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene, whose product, puromycin-N-acetyl-transferase, inactivates puromycin via acetylation.
Puromycin dihydrochloride has been cell culture tested and is recommended for selection of stably transfected cells following transfection with Santa Cruz Biotechnology shRNA or Lentiviral particles. Puromycin dihydrochloride is an antibiotic substance produced by the soil actinomycete Streptomyces alboniger which induces apoptosis in cells by interfering with RNA function, leading to inhibition of protein synthesis. Puromycin dihydrochloride is thought to act as an acyl-tRNA analogue causing premature chain termination. Toxic to both eukaryotic and prokaryotic cells, Puromycin dihydrochloride has also been shown to arrest cells in G2/M phase. 

Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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