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Calcein-AM (CAS: 148504-34-1)
Catalog #:EBC51161
  SKU-Pack Size Availability Size Price
EBC51161-500UG In Stock 500ug £119.90
EBC51161-1MG In Stock 1mg £218.90
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Product Information
Synonym(s) Calcein Acetoxymethyl Ester, NSC 689290
Chemical Name N,N′-[[3′,6′-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1′-Bis[(acetyloxy)methyl] Ester
Application Calcein-AM is a non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule
CAS Number 148504-34-1
Purity ≥96.0%
Molecular Weight 994.86
Molecular Formula C₄₆H₄₆N₂O₂₃
SMILES CC(=O)OCOC(=O)CN(CC1=C(C=CC2=C1OC3=C(C24C5=CC=CC=C5C(=O)O4)C=CC(=C3CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C
Solubility DMSO: 10 mg/ml
Shipping Gel Pack
Storage Store at -20°C
Research Use For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.
Product Description

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to calcein (sc-202090). Calcein-AM is an extremely fluorescent and negatively charged molecule. The Calcein-AM probe can be used to stain living cells and has been observed to fluoresce bright green in cytoplasmic Schwann cells. Calcein-AM has been reported to be a neutral substrate for Mdr (P-glycoprotein (Pgp) and multidrug resistance protein (MRP)) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Studies suggest that Calcein-AM can be used to detect MDR mediated resistance and to be transported by PGP from the plasma membrane. Mitochondrial permeability research has utilized Calcein-AM as a cytosolic fluorophore. Calcein-AM has been used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition. 

Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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