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2-NBDG (CAS: 186689-07-6)
Catalog #£ºEBC51050
  SKU-Pack Size Availability Size Price
EBC51050-5MG 2-4 Weeks 1mg €102.70
EBC51050-10MG 2-4 Weeks 10mg €726.70
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Product Information
Synonym(s) NBD-Glucose, 2-NBD-Glucose
Chemical Name (3R,4R,5S,6R)-6-(hydroxymethyl)-3-((7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)amino)tetrahydro-2H-pyran-2,4,5-triol
Application 2-NBDG is a fluorescent indicator for direct glucose uptake measurement. It is an indicator of cell viability
CAS Number C12H14N4O8
Purity ¡Ý98.0%
Molecular Weight 342.3
Molecular Formula C₁₂H₁₄N₄O₈
SMILES O=C[C@H](NC1=CC=C([N+]([O-])=O)C2=NON=C21)[C@H]([C@@H]([C@@H](CO)O)O)O
Solubility H2O: 5 mg/mL (14.61 mM)
Preparing Stock Solutions
ConcentrationSolventMass 1 mg 5 mg 10 mg
1 mM 2.9218 ml 14.6088 ml 29.2176 ml
5 mM 0.5844 ml 2.9218 ml 5.8435 ml
10 mM 0.2922 ml 1.4609 ml 2.9218 ml
50 mM 0.0584 ml 0.2922 ml 0.5844 ml
Shipping Gel Pack
Storage Store at -20¡ãC
Research Use For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.
Product Description

2-NBDG is a fluorescence-labeled 2-deoxy-glucose analog useful as a tracer for evaluation of cellular glucose metabolism.2-NBDG can be used in many kinds of cells in vitro, such as HepG2 human hepatocarcinoma cells, L6 rat skeletal muscle cells, MCF-7 breast cancer epithelial cells and astrocytes, it is also used in disease models, epilepsy rat, hyperglycemia, diabetes or mouse xenograft model of cancer.2-NBDG enters cells through glucose transporters and is subsequently phosphorylated by hexokinase and trapped inside cells. Flow cytometric detection of fluorescence produced by cells can be performed to examine 2-NBDG uptake into living cells, and the intracellular concentration of transported 2-NBDG can be measured with a fluorescence microplate assay. It can be detected with a fluorescence imaging microscopy or CCD camera simply as well.

Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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