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PMA (CAS: 16561-29-8)

Catalog #：EBC51149

Datasheet

PMA (CAS: 16561-29-8)

SKU-Pack Size	Availability	Size	Price
EBC51149-0.1ML	In Stock	0.1mL(10mg/mL)	€102.70
EBC51149-1MG	In Stock	1mg	€89.70
EBC51149-5MG	In Stock	5mg	€154.70
EBC51149-25MG	In Stock	25mg	€531.70

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Product Information

Synonym(s)

Phorbol-12-myristate 13-acetate

Chemical Name

20-Pentahydroxytiglia-1,6-dien-3-one 12-tetradecanoate 13-acetate

Application

Phorbol-12-Myristate-13-Acetate (PMA) is an activator of ERK/MAPK

CAS Number

16561-29-8

Purity

≥98.0%

Molecular Weight

616.83

Molecular Formula

C₃₆H₅₆O₈

SMILES

CCCCCCCCCCCCCC(O[C@H]([C@H]1C)[C@]2(OC(C)=O)[C@@]([C@@](C=C(CO)C[C@]34O)([H])[C@@]1(O)[C@]4([H])C=C(C)C3=O)([H])C2(C)C)=O

Target & IC50

PKC: EC50 = 11.7 nM

Solubility

DMSO: 100 mg/mL (162.12 mM)
Ethanol: 100 mg/mL (162.12 mM)

Preparing Stock Solutions

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	1.6212 ml	8.1060 ml	16.2119 ml
5 mM	0.3242 ml	1.6212 ml	3.2424 ml
10 mM	0.1621 ml	0.8106 ml	1.6212 ml
50 mM	0.0324 ml	0.1621 ml	0.3242 ml

Shipping

Gel Pack

Storage

Store at -20°C

Research Use

For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.

Product Description

Phorbol-12-Myristate-13-Acetate (PMA) is a diester of phorbol and is a tumor promoting compound extracted from croton oil. PMA is a reversible, highly potent protein kinase C (PKC) activator in vitro and in vivo at nM concentrations. PMA′s effects on PKC are attributed to its similarity to diacylgylerol, a natural activator of PKC. PMA activates Ca2+-ATPase and potentiates forskolin-induced cAMP formation. PMA has been shown to inhibit apoptosis induced by the Fas antigen, but PMA induces apoptosis in HL-60 promyelocytic leukemia cells. PMA is also known as Phorbol-12-Myristate-13-Acetate, TPA, 12-O-Tetradecanoylphorbol-13-acetate, PKC Activator I, and tetradecanoic acid, (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-9a-(acetyloxy)-1a,1b,4,4a,5,7a,7b,8,9,9a-decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9-yl ester.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol