Stains, Dyes and Fluorescent Reagents - Calcein-AM (CAS: 148504-34-1)

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Calcein-AM (CAS: 148504-34-1)

Catalog #：EBC51161

Calcein-AM (CAS: 148504-34-1)

SKU-Pack Size	Availability	Size	Price
EBC51161-500UG	In Stock	500ug	€141.70
EBC51161-1MG	In Stock	1mg	€258.70

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Product Information
Synonym(s)	Calcein Acetoxymethyl Ester, NSC 689290
Chemical Name	N,N′-[[3′,6′-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1′-Bis[(acetyloxy)methyl] Ester
Application	Calcein-AM is a non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule
CAS Number	148504-34-1
Purity	≥96.0%
Molecular Weight	994.86
Molecular Formula	C₄₆H₄₆N₂O₂₃
SMILES	CC(=O)OCOC(=O)CN(CC1=C(C=CC2=C1OC3=C(C24C5=CC=CC=C5C(=O)O4)C=CC(=C3CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C
Solubility	DMSO: 10 mg/ml
Shipping	Gel Pack
Storage	Store at -20°C
Research Use	For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.

Product Description

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to calcein (sc-202090). Calcein-AM is an extremely fluorescent and negatively charged molecule. The Calcein-AM probe can be used to stain living cells and has been observed to fluoresce bright green in cytoplasmic Schwann cells. Calcein-AM has been reported to be a neutral substrate for Mdr (P-glycoprotein (Pgp) and multidrug resistance protein (MRP)) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. Studies suggest that Calcein-AM can be used to detect MDR mediated resistance and to be transported by PGP from the plasma membrane. Mitochondrial permeability research has utilized Calcein-AM as a cytosolic fluorophore. Calcein-AM has been used as a cytosolic fluorophore in mitochondrial permeability studies to image pore transition.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol