Protein Inhibitors - ID-8 (CAS: 147591-46-6)

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ID-8 (CAS: 147591-46-6)

Catalog #：EBC51109

Datasheet

ID-8 (CAS: 147591-46-6)

SKU-Pack Size	Availability	Size	Price
EBC51109-1ML	In Stock	1mL(10mM in DMSO)	￡53.90
EBC51109-5MG	In Stock	5mg	￡64.90
EBC51109-10MG	In Stock	10mg	￡75.90
EBC51109-25MG	In Stock	25mg	￡141.90
EBC51109-50MG	In Stock	50mg	￡240.90

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Product Information

Synonym(s)

HY-15838, ID8, DYRK inhibitor

Chemical Name

1-(4-methoxyphenyl)-2-methyl-3-nitroindol-6-ol

Application

ID-8 is an inhibitor of DYRK

CAS Number

147591-46-6

Purity

≥98.0%

Molecular Weight

298.29

Molecular Formula

C₁₆H₁₄N₂O₄

SMILES

OC1=CC2=C(C=C1)C([N+]([O-])=O)=C(C)N2C3=CC=C(OC)C=C3

Target & IC50

DYRK

Solubility

DMSO: 29.8 mg/mL (100 mM)

Preparing Stock Solutions

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	3.3524 ml	16.7622 ml	33.5244 ml
5 mM	0.6705 ml	3.3524 ml	6.7049 ml
10 mM	0.3352 ml	1.6762 ml	3.3524 ml
50 mM	0.0670 ml	0.3352 ml	0.6705 ml

Shipping

Gel Pack

Storage

Store at -20°C

Research Use

For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.

Product Description

ID-8 is a small molecule DYRK inhibitor. In HES2 cells, ID-8 (0.5 μM) increased hESC survival by 1.1%. The combination of ID-8 and Wnt3a increased survival by 1.7% and completely inhibited Wnt-induced morphological differentiation. Also, ID-8 significantly reduced the expression of differentiation marker gene GATA6, GSC, SOX17 and CDX2 induced by Wnt. In hESCs, ID-8 increased Wnt-mediated hESC proliferation and survival via inhibition of DYRKs. Also, DYRK family is direct targets of ID-8. In embryonic stem cells (ESCs), ID-8 (10 μM) stimulated proliferation in culture without MEFs, serum or LIF. The ID-8 induced ESCs showed the ability to form all three germ layer-derived tissues, such as liver, neurons and muscles. Also, ID-8 increased ESC differentiation and the levels of Nanog, Sox2 and Rex-1 . In mouse ESCs cultured in serum-free medium, ID-8 maintained their self-renewal and pluripotency.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol