Protein Inhibitors - EX 527 (CAS: 49843-98-3)

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EX 527 (CAS: 49843-98-3)

Catalog #：EBC51040

EX 527 (CAS: 49843-98-3)

SKU-Pack Size	Availability	Size	Price
EBC51040-1ML	In Stock	1mL(10mM in DMSO)	~~$73.50~~ / $51.45
EBC51040-5MG	In Stock	5mg	~~$73.50~~ / $51.45
EBC51040-10MG	In Stock	10mg	~~$103.50~~ / $72.45
EBC51040-25MG	In Stock	25mg	~~$163.50~~ / $114.45
EBC51040-50MG	In Stock	50mg	~~$253.50~~ / $177.45

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Product Information

Synonym(s)

EX-527, SEN0014196, Selisistat

Chemical Name

6-Chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide

Application

EX 527 is a potent inhibitor of the SIRT1 class III histone deacetylase enzyme, selective over SIRT2 and SIRT3.

CAS Number

49843-98-3

Purity

≥98.0%

Molecular Weight

248.71

Molecular Formula

C₁₃H₁₃ClN₂O

SMILES

O=C(C1C(NC2=C3C=C(Cl)C=C2)=C3CCC1)N

Target & IC50

SIRT1: IC50 = 98 nM
SIRT2: IC50 = 19.6 μM
SIRT3: IC50 = 48.7 μM

Solubility

DMSO: 49 mg/mL (197.02 mM)
Ethanol: 30 mg/mL (120.62 mM)

Preparing Stock Solutions

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	4.0211 ml	20.1037 ml	40.2075 ml
5 mM	0.8042 ml	4.0207 ml	8.0415 ml
10 mM	0.4021 ml	2.0104 ml	4.0208 ml
50 mM	0.0804 ml	0.4021 ml	0.8042 ml

Shipping

Gel Pack

Storage

Store at 4° C

Research Use

For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.

Product Description

Selisistat (EX-527) is an inhibitor of SIRT1 enzymatic activity (IC50, 98 nM), identified in a high-throughput screen using bacterially expressed human SIRT1. Selisistat (EX-527) inhibits SIRT1 in a concentration-dependent manner with an IC50 of 38 nM, in agreement with the activity on bacterially expressed SIRT1. Selisistat (EX-527) has much lower potency against SIRT2 (IC50, 19.6 μM) or SIRT3 (IC50, 48.7 μM) but does not inhibit class I/II HDAC activity at concentrations up to 100 μM. Selisistat (EX-527) exerts an inhibitory effect on SIRT1 activity without affecting SIRT1 expression on both mRNA and protein levels.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol