Protein Inhibitors - Nutlin-3 (CAS: 548472-68-0)

Nutlin-3 (CAS: 548472-68-0)

Catalog #：EBC51021

Nutlin-3 (CAS: 548472-68-0)

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Product Information

Synonym(s)

Nutlin3, p53/MDM2 Agonist

Chemical Name

4-(4,5-bis(4-chlorophenyl)-2-(2-isopropoxy-4-methoxyphenyl)-4,5-dihydro-1H-imidazole-1-carbonyl)piperazin-2-one

Application

Nutlin-3 is an apoptosis inducer and disruptor of MDM2-p53

CAS Number

548472-68-0

Purity

≥99.0%

Molecular Weight

581.49

Molecular Formula

C₃₀H₃₀Cl₂N₄O₄

SMILES

CC(C)OC1=C(C=CC(=C1)OC)C2=NC(C(N2C(=O)N3CCNC(=O)C3)C4=CC=C(C=C4)Cl)C5=CC=C(C=C5)Cl

Target & IC50

p53/MDM2 interaction: IC50 = 0.09 μM; 1043SK: IC50 = 2.2 μM (human); NIH/3T3: IC50 = 1.3 μM (mouse)

Solubility

DMSO:100 mg/mL (171.97 mM)
Ethanol:100 mg/mL (171.97 mM)

Preparing Stock Solutions

Shipping

Gel Pack

Storage

Store at -20°C

Research Use

For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.

Product Description
Nutlin-3 is an inhibitor of the MDM2-p53 interaction. In particular, co-treatment of p53-positive HCT116 cells with 1 μM of Inauhzin and 2 μM of Nutlin-3 more significantly activated p53 as measured by its protein level as well as the level of its target p21, PUMA or cleaved PARP as indication of apoptosis

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol