Protein Agonists, Antagonists and Activators - Actinomycin D (CAS: 50-76-0)

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Actinomycin D (CAS: 50-76-0)

Catalog #：EBC51004

Datasheet

Actinomycin D (CAS: 50-76-0)

SKU-Pack Size	Availability	Size	Price
EBC51004-1ML	In Stock	1mL(10mM in DMSO)	€310.70
EBC51004-5MG	In Stock	5mg	€76.70
EBC51004-10MG	In Stock	10mg	€115.70
EBC51004-50MG	In Stock	50mg	€466.70

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Product Information

Synonym(s)

Dactinomycin, Act D, RASP-101, Oncostatin K

Chemical Name

2-amino-4,6-dimethyl-3-oxo-1-N,9-N-bis[7,11,14-trimethyl-2,5,9,12,15-pentaoxo-3,10-di(propan-2-yl)-8-oxa-1,4,11,14-tetrazabicyclo[14.3.0]nonadecan-6-yl]phenoxazine-1,9-dicarboxamide

Application

Actinomycin D is a metabolite, apoptosis inducer, and potent antibiotic that binds to the GpC steps of DNA

CAS Number

50-76-0

Purity

≥98%

Molecular Weight

1255.42

Molecular Formula

C₆₂H₈₆N₁₂O₁₆

SMILES

CC1C(C(=O)NC(C(=O)N2CCCC2C(=O)N(CC(=O)N(C(C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=C4C(=C(C=C3)C)OC5=C(C(=O)C(=C(C5=N4)C(=O)NC6C(OC(=O)C(N(C(=O)CN(C(=O)C7CCCN7C(=O)C(NC6=O)C(C)C)C)C)C(C)C)C)N)C

Target & IC50

cell cycle: IC50=0.4 nM
DNA repair: IC50=0.42 nM

Solubility

DMSO: 100 mg/mL (79.65 mM)

Preparing Stock Solutions

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	0.7966 ml	3.9828 ml	7.9656 ml
5 mM	0.1593 ml	0.7966 ml	1.5931 ml
10 mM	0.0797 ml	0.3983 ml	0.7966 ml
50 mM	0.0159 ml	0.0797 ml	0.1593 ml

Shipping

Gel Pack

Storage

Store at -20°C

Research Use

For Research Use Only. Not Intended for Diagnostic Or Therapeutic Use.

Product Description

Actinomycin D is a metabolite isolated from Streptomyces parvulus. Actinomycin D binds to the GpC steps of DNA. Actinomycin D acts as an antibiotic, and is more effective against gram positive bacteria than gram negative bacteria. Actinomycin D can induce apoptosis in several cell lines such as lung and prostate cancer lines, and human blood lymphocytes, among others. In studies, it has been observed that Actinomycin D elevates p53 expression, leading to the increase of PUMAα/β (the p53 upregulated modulator of apoptosis protein; PUMA). Actinomycin D can also induce tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced caspase-dependent and -independent apoptosis.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol