A-769662 is an AMP-activated protein kinase (AMPK) activator that mimics the function of AMP on AMPK β-1 by allosteric activation and the inhibition of dephosphorylation of AMPK. In the prescence of both LKB1 and A-769662, the phosphorylation of AMPK is suppressed. In addition, A-769662 is not effected by upstream kinases such as calmodulin-dependent protein kinase kinase-β when phosphorylating AMPK beta-1. In vitro tests indicate A-769662 suppresses the activity of Sodium-Potassium Adenosine Triphosphatase by inhibiting the α(1)-isoform of the pump and decreasing the cell surface abundance of the protein complex in skeletal muscle cells and induces glucose uptake through a PI3-kinase-dependent pathway.