SKU-Pack Size | Availability | Size | Price | |
EAB10004-100UL | In Stock | 100ul | $268.50 | |
EAB10004-200UL | In Stock | 200ul | $418.50 |
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EbioCell | Phone:(540) 808-3925 |
Imperial Business Park 4819 Emperor Boulevard, | E-mail:order@ebiocell.com | |
Canada | Suite 408 Durham, NC 27703, USA | Web:www.ebiocell.com |
Product Information | |
Applications | WB, IHC-P, IF/ICC |
Species Reactivity | Human, Mouse, Rat, Monkey, Dog, Pig, Bovine, Chicken, Hamster, Sheep |
Host / Isotype | Rabbit IgG |
Clonality | Polyclonal |
Applications Dilutions | WB=1:5000-20000 IHC-P=1:50-300 IF/ICC=1:50-300 |
MW (kDa) | 37 |
Conjugate | Unconjugate |
Specificity | GAPDH Rabbit Polyclonal Antibody detects endogenous levels of GAPDH protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
Product Bioinformatics | |
Synonym(s) | Glyceraldehyde-3-phosphate dehydrogenase; GAPDH |
Gene Aliases | GAPDH |
UniProt ID | |
Entrez Gene ID |
Product Description | |
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. |
Product Image Gallery | |
![]() Western blot analysis of extracts on different cells, using GAPDH Rabbit Polyclonal Antibody (EAB10004) at 1:20000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: Hela Lane2: NIH/3T3 Lane3: 293T Lane4: Rat brain Lane5: Mouse brain |
Specific Protocols | |