SKU-Pack Size | Availability | Size | Price | |
EAB22302-100UL | In Stock | 100ul | ¥2290.00 |
Please select the country you are in to find your local distributor. |
北京美瑞克生物科技有限公司 | Phone: +86 010-62890160 / 13691184142(微信同号) | |
2 / F, 128 malianwa North Road, Haidian District, | E-mail: mrkbio@163.com | |
China | Beijing China | Website: www.mrkbio.com |
Product Information | |
Applications | WB, IP, IF/ICC |
Species Reactivity | All |
Host / Isotype | Rabbit IgG |
Clonality | Monoclonal |
Applications Dilutions | WB=1:500-2000 IP=1:10-100 IF/ICC=1:50-200 |
MW (kDa) | 82 |
Conjugate | Unconjugate |
Specificity | SV40 Large T Antigen Rabbit Monoclonal Antibody detects endogenous levels of SV40 Large T Antigen protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
Product Bioinformatics | |
Synonym(s) | Large T antigen; SV40TAg; SV40gp6; LT-AG |
Gene Aliases | SV40GP6 |
UniProt ID | |
Entrez Gene ID |
Product Description | |
Simian virus SV40 has provided an important model for studies of cellular mechanisms involved in a malignant transformation. The major SV40 translational products include the large T antigen and the small T antigen, both of which are encoded by the early region of the SV40 viral ge-nome. The large T antigen complexes with the p53 suppressor gene, resulting in its functional inactivation, thus promoting cell transformation. In addition, SV40 large T antigen binds DNA polymerase and the transcription factor AP-2. It also forms complexes with a second tumor supressor gene-encoded protein, Rb 105. Binding of SV40 T antigen is specific for the "pocket" domain of Rb p105, which is also the binding site for the E2F cellular transcription factor. |
Product Image Gallery | |
Western blot analysis of extracts on different cells, using SV40 Large T Antigen Rabbit Monoclonal Antibody (EAB22302) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: HL60 Lane2: A549 |
Specific Protocols | |