Monoclonal Antibodies - MCM3 Mouse Monoclonal Antibody

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MCM3 Mouse Monoclonal Antibody

Catalog #：EAB23003

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB23003-30UL	In Stock	30ul	€115.70
EAB23003-100UL	In Stock	100ul	€258.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Cambiotech	Phone：+44 (0) 188 448801
	Lanwades Business Park,	E-mail：info@cambiotech.com
Ireland	Kobenhavn K, Ireland	Web：www.cambiotech.com

Product Information
Applications	WB, IHC-P, IF/ICC, FC, ELISA
Species Reactivity	Human, Rat, Monkey
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:50-200　 FC=1:50-200
MW (kDa)	91
Conjugate	Unconjugate
Specificity	MCM3 Mouse Monoclonal Antibody detects endogenous levels of MCM3 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Minichromosome maintenance complex component 3; HCC5; P1.h; RLFB; P1-MCM3; MCM3
Gene Aliases	MCM3
UniProt ID	P25205
Entrez Gene ID	4172

Product Description

MCM3 is a member of the mini-chromosome maintenance (MCM) protein family. MCM3 plays a crucial role in the regulation of DNA replication, ensuring that DNA is replicated only once per cell cycle. MCM3 is located in the nucleus, where MCM3 forms part of the MCM2-7 complex, which acts as a helicase to unwind DNA during replication. The proper functioning of MCM3 and associated proteins is essential for the initiation of DNA synthesis, and any dysregulation can have significant implications for cell proliferation and tumorigenesis. MCM3 is also subject to post-translational modifications, which can influence MCM3 activity and interactions with other proteins, further underscoring MCM3′s importance in cellular processes.

Product Image Gallery

Western blot analysis of extracts on Hela, PC12, COS7, HepG2 cells, using MCM3 Mouse Monoclonal Antibody (EAB23003) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue, using MCM3 Mouse Monoclonal Antibody (EAB23003) at 1:200 dilution.

Immunofluorescence analysis of Hela cell, using MCM3 Mouse Monoclonal Antibody (EAB23003)(green) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol