Polyclonal Antibodies - WASH1 Rabbit Polyclonal Antibody

Location：Home > Antibodies > Primary Antibodies > Polyclonal Antibodies

WASH1 Rabbit Polyclonal Antibody

Catalog #：EAB12154

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB12154-30UL	In Stock	30ul	$133.50
EAB12154-100UL	In Stock	100ul	$298.50
EAB12154-200UL	In Stock	200ul	$508.50

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
Canada	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000
MW (kDa)	50
Conjugate	Unconjugate
Specificity	WASH1 Rabbit Polyclonal Antibody detects endogenous levels of WASH1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	WASH complex subunit 1; WASH; WASH1; FAM39E; WASHC1
Gene Aliases	WASHC1
UniProt ID	A8K0Z3
Entrez Gene ID	100287171

Product Description

WASH1 also known as WASHC1 is a protein coding gene with endocytosis as a related pathway. Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Its assembly in the WASH core complex seems to inhibit its NPF activity and via WASHC2 is required for its membrane targeting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Enables alpha-tubulin binding activity and ubiquitin protein ligase binding activity. Located in several cellular components, including endosome; exocyst; and lamellipodium. Part of WASH complex.

Product Image Gallery
Western blot analysis of extracts on U2OS cells, using WASH1 Rabbit Polyclonal Antibody (EAB12154) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution. Predicted band size: 50 kDa; Observed band size: 50-70 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol