Recombinant Antibodies - CDT1 Rabbit Monoclonal Antibody

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CDT1 Rabbit Monoclonal Antibody

Catalog #：EAB22834

	SKU-Pack Size	Availability	Size	Price
	EAB22834-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	OlaSciences	Phone：+33 (0) 9 77 4001 556
	87, rue des Suisses 65000 Nanterre,	E-mail：ola@olasciences.com
France	France	Web：www.olasciences.com

Product Information
Applications	WB, IP, IHC-P, IF/ICC
Species Reactivity	Human
Host / Isotype	Rabbit IgG
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF/ICC=1:50-300　 IP=1:20-200 FC=1:10-100
MW (kDa)	60
Conjugate	Unconjugate
Specificity	CDT1 Rabbit Monoclonal Antibody detects endogenous levels of CDT1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Chromatin licensing and DNA replication factor 1; DUP; RIS2; CDT1
Gene Aliases	CDT1
UniProt ID	Q9H211
Entrez Gene ID	81620

Product Description

CDT1 also known as DUP is involved in the formation of the pre-replication complex that is necessary for DNA replication. CDT1 is a nuclear-localizing replication initiation factor, specifically expressed during the G1 and S phases of the cell cycle. CDT1 facilitates the loading of the minichromosome maintenance (MCM) complex onto chromatin at the end of mitosis, a critical step necessary for DNA replication initiation. While CDT1 mRNA maintains consistent expression throughout the cell cycle, CDT1 levels decrease significantly after S-phase onset, indicating proteolytic degradation′s essential role in maintaining proper levels and preventing untimely DNA replication. CDT1 also interacts with DNA replication inhibitor geminin during S and G2 phases, preventing inappropriate origin firing. CDT1′s ability to reverse geminin′s inhibition of DNA replication in cell-free extracts highlights CDT1′s significance in DNA synthesis and cell cycle progression regulation.

Product Image Gallery
Western blot analysis of extracts on Hela, HepG2, Jurkat, SH-SY5Y cells, using CDT1 Rabbit Monoclonal Antibody (EAB22834) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol