Monoclonal Antibodies - PPARα Mouse Monoclonal Antibody

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PPARα Mouse Monoclonal Antibody

Catalog #：EAB22127

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22127-100UL	In Stock	100ul	€336.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	OlaSciences	Phone：+33 (0) 9 77 4001 556
	87, rue des Suisses 65000 Nanterre,	E-mail：ola@olasciences.com
France	France	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IHC-P=1:100-500　 IF/ICC=1:100-500
MW (kDa)	52
Conjugate	Unconjugate
Specificity	PPARα Mouse Monoclonal Antibody detects endogenous levels of PPARα protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Peroxisome proliferator activated receptor alpha; PPAR; NR1C1; hPPAR; PPARalpha; PPAR-alpha; PPARA
Gene Aliases	PPARA
UniProt ID	Q07869
Entrez Gene ID	5465

Product Description

PPARα also known as PPARalpha, PPARA is a pivotal nuclear hormone receptor that plays a significant role in regulating fatty acid metabolism, lipid homeostasis, and overall energy balance, making PPARα essential for maintaining metabolic health. By forming heterodimers with retinoid X receptors (RXRs), PPARα binds to specific PPAR-response elements (PPREs) in DNA, thereby modulating the transcription of genes involved in lipid metabolism and energy utilization. PPARα functionality is crucial in primary hepatocytes, where PPARα governs the expression of proteins necessary for fatty acid oxidation and overall metabolic processes. Additionally, PPARα interacts with other nuclear receptors such as LXRα and thyroid receptors, which can act as antagonists to PPARα binding with RXRα at PPREs, highlighting the intricate regulatory mechanisms governing lipid metabolism.

Product Image Gallery
Western blot analysis of extracts on HepG2, HSCT6 cells, using PPARα Mouse Monoclonal Antibody (EAB22127) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol