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Location：Home > Antibodies > Primary Antibodies > Recombinant Antibodies
GEF-H1 Rabbit Monoclonal Antibody
Catalog #：EAB22192
Datasheet
  SKU-Pack Size Availability Size Price
EAB22192-100UL In Stock 100ul ￥2290.00
If you want to get a larger packsize, Please contact your local distributor or
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2 / F, 128 malianwa North Road, Haidian District, E-mail：   mrkbio@163.com 
China Beijing China Website： www.mrkbio.com
Product Information
Applications WB, IF/ICC, FC
Species Reactivity Human, Mouse, Rat
Host / Isotype Rabbit IgG
Clonality Monoclonal
Applications Dilutions WB=1:500-2000　
IF/ICC=1:50-200
FC=1:10-100
MW (kDa) 112
Conjugate Unconjugate
Specificity GEF-H1 Rabbit Monoclonal Antibody detects endogenous levels of GEF-H1 protein.
Purification Affinity purification
Concentration 1mg/ml
Format Liquid
Formulation In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping Gel Pack
Storage Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin
IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key

H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish
Hr-Horse All-All Species Expected
Product Bioinformatics
Synonym(s) Rho/Rac guanine nucleotide exchange factor 2; GEF; Lfc; P40; GEFH1; LFP40; GEF-H1; NEDMHM; ARHGEF2
Gene Aliases ARHGEF2
UniProt ID

Q92974
Entrez Gene ID

9181
Product Description

GEF-H1 also known as Lfc, ARHGEF2 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization. Loss of interaction between GEF-H1 and microtubules leads to activation of Rho. Phosphorylation of GEF-H1 at Ser886, a site located in the 14-3-3 binding motif, has been implicated in recruitment of 14-3-3 and GEF-H1 to microtubules, and in the regulation of RhoA activity in response to mitotic kinases during cytokinesis.GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability. GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest.  
Product Image Gallery
Western blot analysis of extracts on Hela, HEK293, THP-1 cells, using GEF-H1 Rabbit Monoclonal Antibody (EAB22192) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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