Recombinant Antibodies - GEF-H1 Rabbit Monoclonal Antibody

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GEF-H1 Rabbit Monoclonal Antibody

Catalog #：EAB22192

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22192-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Dianost	Phone：+49 (0) 40 65472-053
	Bullenkoppel 11， 23896 Poggensee,	E-mail：ola@olasciences.com
Germany	Germany	Web：www.olasciences.com

Product Information
Applications	WB, IF/ICC, FC
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IF/ICC=1:50-200 FC=1:10-100
MW (kDa)	112
Conjugate	Unconjugate
Specificity	GEF-H1 Rabbit Monoclonal Antibody detects endogenous levels of GEF-H1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Rho/Rac guanine nucleotide exchange factor 2; GEF; Lfc; P40; GEFH1; LFP40; GEF-H1; NEDMHM; ARHGEF2
Gene Aliases	ARHGEF2
UniProt ID	Q92974
Entrez Gene ID	9181

Product Description

GEF-H1 also known as Lfc, ARHGEF2 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization. Loss of interaction between GEF-H1 and microtubules leads to activation of Rho. Phosphorylation of GEF-H1 at Ser886, a site located in the 14-3-3 binding motif, has been implicated in recruitment of 14-3-3 and GEF-H1 to microtubules, and in the regulation of RhoA activity in response to mitotic kinases during cytokinesis.GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability. GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest.

Product Image Gallery
Western blot analysis of extracts on Hela, HEK293, THP-1 cells, using GEF-H1 Rabbit Monoclonal Antibody (EAB22192) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol