Monoclonal Antibodies - Vinculin Mouse Monoclonal Antibody

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Vinculin Mouse Monoclonal Antibody

Catalog #：EAB21173

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21173-100UL	In Stock	100ul	$388.50

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat, Rabbit
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:5000-20000　 IHC-P=1:500-2000　 IF/ICC=1:500-2000
MW (kDa)	124
Conjugate	Unconjugate
Specificity	Vinculin Mouse Monoclonal Antibody detects endogenous levels of Vinculin protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Vinculin; MV; MVCL; VINC; CMD1W; CMH15; HEL114; VCL
Gene Aliases	VCL
UniProt ID	P18206
Entrez Gene ID	7414

Product Description

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin plays a crucial role in cellular adhesion and mechanotransduction, functioning as a key component of focal adhesions, which are specialized structures that connect the cytoskeleton to the extracellular matrix. These focal adhesions are essential for maintaining cell shape, enabling cell migration, and facilitating communication between the cell and its environment. Vinculin′s ability to interact with other cytoskeletal proteins, such as Talin and α-actinin, underscores vinculin′s importance in linking the actin cytoskeleton to integrins in the plasma membrane, thereby influencing cellular responses to mechanical stimuli.

Product Image Gallery
Western blot analysis of extracts on HuvEc, K562, Mouse heart, Rat heart cells, using Vinculin Mouse Monoclonal Antibody (EAB21173) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol