Monoclonal Antibodies - Chk1 Mouse Monoclonal Antibody

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Chk1 Mouse Monoclonal Antibody

Catalog #：EAB21409

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21409-100UL	In Stock	100ul	￡218.90

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email us at info@ebiocell.com

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, ELISA
Species Reactivity	Human, Mouse
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000
MW (kDa)	54
Conjugate	Unconjugate
Specificity	Chk1 Mouse Monoclonal Antibody detects endogenous levels of Chk1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Checkpoint kinase 1; CHK1; OZEMA21; CHEK1
Gene Aliases	CHEK1
UniProt ID	O14757
Entrez Gene ID	1111

Product Description

Chk1 also known as CHEK1 plays a critical role in the cellular response to DNA damage, functioning as a key regulator in the G2 DNA damage checkpoint. By phosphorylating Cdc25C, Chk1 inhibits its activity, which in turn prevents the activation of cyclin-dependent kinases and blocks the progression of mitosis. This regulatory mechanism is vital for maintaining genomic stability, as cells can repair DNA damage before entering mitosis, thereby preventing the propagation of damaged DNA to daughter cells. Chk1 has been shown to interact with other proteins involved in cell cycle regulation, including Wee1, further underscoring Chk1′s importance in the intricate network of cell cycle control.

Product Image Gallery
Western blot analysis of extracts on Hela, A431, K562, NIH/3T3 cells, using Chk1 Mouse Monoclonal Antibody (EAB21409) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol