Monoclonal Antibodies - TRIM25 Mouse Monoclonal Antibody

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TRIM25 Mouse Monoclonal Antibody

Catalog #：EAB22285

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22285-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IHC-P, IF/ICC, FC, ELISA
Species Reactivity	Human
Host / Isotype	Mouse IgG2b
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:100-500 FC=1:50-200
MW (kDa)	71
Conjugate	Unconjugate
Specificity	TRIM25 Mouse Monoclonal Antibody detects endogenous levels of TIA1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Tripartite motif containing 25; EFP; Z147; RNF147; ZNF147; TRIM25
Gene Aliases	TRIM25
UniProt ID	Q14258
Entrez Gene ID	7706

Product Description

TRIM25 also known as Z147, RNF147, ZNF147, EFP is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The structural composition of EFP allows for precise interaction with estrogen receptors, facilitating transcriptional regulation of target genes in response to estrogen signaling. This interaction is vital for growth, differentiation, and function of various tissues, highlighting EFP′s importance in mediating estrogen′s effects. The regulation of EFP gene expression involves multiple promoter elements and interacting factors, which underscores EFP′s role in a broader signaling cascade that influences secondary estrogen-responsive genes.

Product Image Gallery
Western blot analysis of extracts on A549, K562, Molt-4, MCF7 cells, using TRIM25 Mouse Monoclonal Antibody (EAB22285) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol