Monoclonal Antibodies - Occludin Mouse Monoclonal antibody

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Occludin Mouse Monoclonal antibody

Catalog #：EAB22081

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22081-100UL	In Stock	100ul	￡284.90

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, IHC-P, ELISA
Species Reactivity	Human, Mouse, Rat, Pig, Bovine
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:5000-20000　 IHC-P=1:100-500
MW (kDa)	59
Conjugate	Unconjugate
Specificity	Occludin Mouse Monoclonal antibody detects endogenous levels of Occludin protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Occludin; BLCPMG; PTORCH1; PPP1R115; OCLN
Gene Aliases	OCLN
UniProt ID	Q16625
Entrez Gene ID	100506658

Product Description

Occludin also known as OCLN is an integral membrane protein that plays a crucial role in forming and maintaining tight junctions in epithelial and endothelial cells, which are essential for regulating paracellular permeability and maintaining blood-brain barrier integrity. Occludin features a tetraspan structure with two extracellular loops that facilitate interactions with other tight junction proteins, contributing to the overall architecture and function of the tight junction complex. Post-translational modifications, including phosphorylation, are vital for Occludin localization and functional regulation. Phosphorylation of Occludin can influence tight junction formation, impacting cellular permeability to large molecules.

Product Image Gallery
Western blot analysis of extracts on HuvEC, U2OS, HEK293, Mouse colon cells, using Occludin Mouse Monoclonal antibody (EAB22081) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol