Monoclonal Antibodies - AATF Mouse Monoclonal Antibody

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AATF Mouse Monoclonal Antibody

Catalog #：EAB22121

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22121-100UL	In Stock	100ul	$388.50

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:5000-20000　 IHC-P=1:100-500
MW (kDa)	63
Conjugate	Unconjugate
Specificity	AATF Mouse Monoclonal Antibody detects endogenous levels of AATF protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Apoptosis antagonizing transcription factor; DED; BFR2; CHE1; CHE-1; AATF
Gene Aliases	AATF
UniProt ID	Q9NY61
Entrez Gene ID	26574

Product Description

AATF(Apoptosis-antagonizing transcription factor) also known as Che-1 is a widely expressed nuclear protein that belongs to the AATF family. Hyperphosphorylated during the G1/S phase transition, AATF may function as a general inhibitor of the histone deacetylase HDAC1. AATF binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of AATF.

Product Image Gallery
Western blot analysis of extracts on Hela, Jurkat, HepG2, K562 cells, using AATF Mouse Monoclonal Antibody (EAB22121) at 1:5000 dilution. Secondary antibody Goat Anti-RMouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol