SKU-Pack Size | Availability | Size | Price | |
EAB11845-30UL | In Stock | 30ul | €115.70 | |
EAB11845-100UL | In Stock | 100ul | €258.70 | |
EAB11845-200UL | In Stock | 200ul | €440.70 |
Please select the country you are in to find your local distributor. |
Betaclonal | Phone£º+34 (0)91 471 6763 | |
29/1 Lomonosovsky Pr, | E-mail£ºtech@betaclonal.com | |
Spain | Madrid, Spain | Web£ºwww.betaclonal.com |
Product Information | |
Applications | WB, IHC-P, IF, ELISA |
Species Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit IgG |
Clonality | Polyclonal |
Applications Dilutions | WB=1:500-2000¡¡ IHC-P=1:50-300¡¡ IF=1:50-300 |
MW (kDa) | 63 |
Conjugate | Unconjugate |
Specificity | AATF Rabbit Polyclonal Antibody detects endogenous levels of AATF protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20¡ãC least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4¡ãC for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
Product Bioinformatics | |
Synonym(s) | Apoptosis antagonizing transcription factor; DED; BFR2; CHE1; CHE-1; AATF |
Gene Aliases | AATF |
UniProt ID | |
Entrez Gene ID |
Product Description | |
AATF(Apoptosis-antagonizing transcription factor) also known as Che-1 is a widely expressed nuclear protein that belongs to the AATF family. Hyperphosphorylated during the G1/S phase transition, AATF may function as a general inhibitor of the histone deacetylase HDAC1. AATF binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of AATF. |
Product Image Gallery | |
Western blot analysis of extracts on HuvEC, Hela, HepG2 cells, using AATF Rabbit Polyclonal Antibody (EAB11845) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using AATF Rabbit Polyclonal Antibody (EAB11845) at 1:200 dilution.
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Specific Protocols | |