Polyclonal Antibodies - AATF Rabbit Polyclonal Antibody

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AATF Rabbit Polyclonal Antibody

Catalog #：EAB11845

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB11845-30UL	In Stock	30ul	€115.70
EAB11845-100UL	In Stock	100ul	€258.70
EAB11845-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	OlaSciences	Phone：+33 (0) 9 77 4001 556
	87, rue des Suisses 65000 Nanterre,	E-mail：ola@olasciences.com
France	France	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF=1:50-300
MW (kDa)	63
Conjugate	Unconjugate
Specificity	AATF Rabbit Polyclonal Antibody detects endogenous levels of AATF protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Apoptosis antagonizing transcription factor; DED; BFR2; CHE1; CHE-1; AATF
Gene Aliases	AATF
UniProt ID	Q9NY61
Entrez Gene ID	26574

Product Description

AATF(Apoptosis-antagonizing transcription factor) also known as Che-1 is a widely expressed nuclear protein that belongs to the AATF family. Hyperphosphorylated during the G1/S phase transition, AATF may function as a general inhibitor of the histone deacetylase HDAC1. AATF binding to the pocket region of Rb may displace HDAC1 from Rb/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Displacement of HDAC1 from Sp1 bound to the p21 promoter leads to increased expression of AATF.

Product Image Gallery
Western blot analysis of extracts on HuvEC, Hela, HepG2 cells, using AATF Rabbit Polyclonal Antibody (EAB11845) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution. Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using AATF Rabbit Polyclonal Antibody (EAB11845) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol