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Location：Home > Antibodies > Primary Antibodies > Polyclonal Antibodies
Phospho-PKR (Thr258) Rabbit Polyclonal Antibody
Catalog #：EAB14077
Datasheet
  SKU-Pack Size Availability Size Price
EAB14077-30UL In Stock 30ul ￥1090.00
EAB14077-100UL In Stock 100ul ￥2290.00
EAB14077-200UL In Stock 200ul ￥3690.00
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Product Information
Applications WB, IHC-P, IF, ELISA
Species Reactivity Human, Mouse, Rat
Host / Isotype Rabbit IgG
Clonality Polyclonal
Applications Dilutions WB=1:500-2000　
IHC-P=1:50-300　
IF=1:50-300
MW (kDa) 62
Conjugate Unconjugate
Specificity Phospho-PKR (Thr258) Rabbit Polyclonal Antibody detects endogenous levels of PKR protein only when phosphorylated at Thr258.
Purification Affinity purification
Concentration 1mg/ml
Format Liquid
Formulation In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping Gel Pack
Storage Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin
IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key

H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish
Hr-Horse All-All Species Expected
Product Bioinformatics
Synonym(s) Eukaryotic translation initiation factor 2 alpha kinase 2; PKR; PRKR; DYT33; LEUDEN; EIF2AK1; PPP1R83; EIF2AK2
Gene Aliases EIF2AK2
UniProt ID

P19525
Entrez Gene ID

5610
Product Description

PKR also known as EIF2AK2 is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses. 
Product Image Gallery
Western blot analysis of extracts on Jurkat cells treated with Starve, using Phospho-PKR (Thr258) Rabbit Polyclonal Antibody (EAB14077) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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