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Location：Home > Antibodies > Primary Antibodies > Monoclonal Antibodies
His-Tag Mouse Monoclonal Antibody
Catalog #：EAB11287
Datasheet
  SKU-Pack Size Availability Size Price
EAB11287-100UL In Stock 100ul ￥1090.00
EAB11287-200UL In Stock 200ul ￥1790.00
If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com
Please select the country you are in to find your local distributor.
北京美瑞克生物科技有限公司 Phone：  +86 010-62890160 / 13691184142(微信同号)
2 / F, 128 malianwa North Road, Haidian District, E-mail：   mrkbio@163.com 
China Beijing China Website： www.mrkbio.com
Product Information
Applications WB, IP, IF/ICC
Species Reactivity N/A
Host / Isotype Mouse IgG1
Clonality Monoclonal
Applications Dilutions WB=1:5000-20000　　
IP=1:100-500 　
IF/ICC=1:500-2000
MW (kDa) N/A
Conjugate Unconjugate
Specificity His-Tag Mouse Monoclonal Antibody detects endogenous levels of His-Tag protein.
Purification Affinity purification
Concentration 1mg/ml
Format Liquid
Formulation In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping Gel Pack
Storage Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin
IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key

H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish
Hr-Horse All-All Species Expected
Product Bioinformatics
Synonym(s) His-Tag; 6His; 6 His Tag; HIS
Gene Aliases HIS
UniProt ID

N/A
Entrez Gene ID

N/A
Product Description

His-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.  
Product Image Gallery
Western blot of HA-Tag fusion protein with His-Tag Mouse Monoclonal Antibody (EAB11287) at 1:5000 and 1:10000 dilution.
Immunofluorescence analysis of 293 cell transfected with a His-Tag protein, using His-Tag Mouse Monoclonal Antibody (EAB11287) at 1:1000 dilution.
Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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