Monoclonal Antibodies - His-Tag Mouse Monoclonal Antibody

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His-Tag Mouse Monoclonal Antibody

Catalog #：EAB11287

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB11287-100UL	In Stock	100ul	€141.70
EAB11287-200UL	In Stock	200ul	€232.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IP, IF/ICC
Species Reactivity	N/A
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:5000-20000　　 IP=1:100-500 　 IF/ICC=1:500-2000
MW (kDa)	N/A
Conjugate	Unconjugate
Specificity	His-Tag Mouse Monoclonal Antibody detects endogenous levels of His-Tag protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	His-Tag; 6His; 6 His Tag; HIS
Gene Aliases	HIS
UniProt ID	N/A
Entrez Gene ID	N/A

Product Description

His-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

Product Image Gallery
Western blot of HA-Tag fusion protein with His-Tag Mouse Monoclonal Antibody (EAB11287) at 1:5000 and 1:10000 dilution. Immunofluorescence analysis of 293 cell transfected with a His-Tag protein, using His-Tag Mouse Monoclonal Antibody (EAB11287) at 1:1000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol