Monoclonal Antibodies - Calreticulin Mouse Monoclonal Antibody

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Calreticulin Mouse Monoclonal Antibody

Catalog #：EAB22495

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22495-100UL	In Stock	100ul	￡251.90

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, IHC-P, IF/ICC, FC, ELISA
Species Reactivity	Human, Mouse, Rat, Rabbit
Host / Isotype	Mouse IgG2a
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:100-500　 FC=1:50-200
MW (kDa)	48
Conjugate	Unconjugate
Specificity	Calreticulin Mouse Monoclonal Antibody detects endogenous levels of Calreticulin protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Calreticulin; RO; CRT; SSA; CALR1; cC1qR; HEL-S-99n; CALR
Gene Aliases	CALR
UniProt ID	P27797
Entrez Gene ID	811

Product Description

Calreticulin also known as CALR is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.

Product Image Gallery

Western blot analysis of extracts on Hela, A549, MCF7 cells, using Calreticulin Mouse Monoclonal Antibody (EAB22495) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Predicted band size: 48 kDa; Observed band size: 55 kDa

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue, using Calreticulin Mouse Monoclonal Antibody (EAB22495) at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue, using Calreticulin Mouse Monoclonal Antibody (EAB22495) at 1:200 dilution.

Immunofluorescence analysis of NIH/3T3 cell, using Calreticulin Mouse Monoclonal Antibody (EAB22495)(green) at 1:100 dilution.

Flow cytometric analysis of NIH/3T3 cell, using Calreticulin Mouse Monoclonal Antibody (EAB22495)(green) at 1:50 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol