Monoclonal Antibodies - CD14 Mouse Monoclonal Antibody

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CD14 Mouse Monoclonal Antibody

Catalog #：EAB21153

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21153-100UL	In Stock	100ul	$343.50

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P, FC, ELISA
Species Reactivity	Human, Mouse, Rat, Rabbit
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:100-500　 FC=1:50-200
MW (kDa)	40
Conjugate	Unconjugate
Specificity	CD14 Mouse Monoclonal Antibody detects endogenous levels of CD14 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	CD14 molecule; CD14
Gene Aliases	CD14
UniProt ID	P08571
Entrez Gene ID	929

Product Description

CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.

Product Image Gallery

Western blot analysis of extracts on Hela, HL60, HepG2, NIH/3T3, HEK293 cells, using Phospho-Mnk1 (Thr255) Rabbit Polyclonal Antibody (EAB21153) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Predicted band size: 40 kDa; Observed band size: 45-55 kDa

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue, using Phospho-Mnk1 (Thr255) Rabbit Polyclonal Antibody (EAB21153) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol