SKU-Pack Size | Availability | Size | Price | |
EAB11513-30UL | In Stock | 30ul | €115.70 | |
EAB11513-100UL | In Stock | 100ul | €258.70 | |
EAB11513-200UL | In Stock | 200ul | €440.70 |
Please select the country you are in to find your local distributor. |
Betaclonal | Phone£º+34 (0)91 471 6763 | |
29/1 Lomonosovsky Pr, | E-mail£ºtech@betaclonal.com | |
Spain | Madrid, Spain | Web£ºwww.betaclonal.com |
Product Information | |
Applications | WB, IHC-P, IF, ELISA |
Species Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit IgG |
Clonality | Polyclonal |
Applications Dilutions | WB=1:500-2000¡¡ IHC-P=1:50-300¡¡ IF=1:50-300 |
MW (kDa) | 37 |
Conjugate | Unconjugate |
Specificity | Bub3 Rabbit Polyclonal Antibody detects endogenous levels of Bub3 protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20¡ãC least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4¡ãC for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
Product Bioinformatics | |
Synonym(s) | BUB3 mitotic checkpoint protein; BUB3L; hBUB3; BUB3 |
Gene Aliases | BUB3 |
UniProt ID | |
Entrez Gene ID |
Product Description | |
Bub3 also known as BUB3L, hBUB3 is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain. |
Product Image Gallery | |
Western blot analysis of extracts on Hela, 293, A549, Jurkat cells, using Bub3 Rabbit Polyclonal Antibody (EAB11513) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 37 kDa; Observed band size: 40 kDa Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using Bub3 Rabbit Polyclonal Antibody (EAB11513) at 1:200 dilution.
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Specific Protocols | |