Monoclonal Antibodies - Brachyury Mouse Monoclonal Antibody

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Brachyury Mouse Monoclonal Antibody

Catalog #：EAB22483

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22483-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Cambiotech	Phone：+44 (0) 188 448801
	Lanwades Business Park,	E-mail：info@cambiotech.com
Ireland	Kobenhavn K, Ireland	Web：www.cambiotech.com

Product Information
Applications	WB, IHC-P, FC, ELISA
Species Reactivity	Human
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 FC=1:50-200
MW (kDa)	47
Conjugate	Unconjugate
Specificity	Brachyury Mouse Monoclonal Antibody detects endogenous levels of Brachyury protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	T-box transcription factor T; T; TFT; SAVA; TBXT
Gene Aliases	TBXT
UniProt ID	O15178
Entrez Gene ID	6862

Product Description

Brachyury also known as T, TFT, TBXT is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies.

Product Image Gallery
Western blot analysis of extracts on Raji, Jurkat cells, using Brachyury Mouse Monoclonal Antibody (EAB22483) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue, using Brachyury Mouse Monoclonal Antibody (EAB22483) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol