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Location：Home > Antibodies > Primary Antibodies > Monoclonal Antibodies
Brd4 Mouse Monoclonal Antibody
Catalog #：EAB22485
Datasheet
  SKU-Pack Size Availability Size Price
EAB22485-100UL In Stock 100ul ￥2590.00
If you want to get a larger packsize, Please contact your local distributor or
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北京美瑞克生物科技有限公司 Phone：  +86 010-62890160 / 13691184142(微信同号)
2 / F, 128 malianwa North Road, Haidian District, E-mail：   mrkbio@163.com 
China Beijing China Website： www.mrkbio.com
Product Information
Applications WB, IP, ChIP
Species Reactivity Human, Mouse
Host / Isotype Mouse IgG1
Clonality Monoclonal
Applications Dilutions WB=1:1000-5000　　
ChIP=1:100-500
MW (kDa) 152
Conjugate Unconjugate
Specificity Brd4 Mouse Monoclonal Antibody detects endogenous levels of Brd4 protein.
Purification Affinity purification
Concentration 1mg/ml
Format Liquid
Formulation In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping Gel Pack
Storage Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin
IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key

H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish
Hr-Horse All-All Species Expected
Product Bioinformatics
Synonym(s) Bromodomain containing 4; CAP; MCAP; CDLS6; HUNK1; HUNKI; FSHRG4; BRD4
Gene Aliases BRD4
UniProt ID

O60885
Entrez Gene ID

23476
Product Description

Brd4 belongs to the BET family, a group of structurally related proteins containing two bromodomains. Through these two domains, Brd4 associates with mitotic chromosomes and its expression correlates with cell growth. Expression of Brd4 inhibits cell cycle progression from G1 to S, due to binding to the largest subunit of replication factor C (RFC) to prevent DNA elongation. Altered Brd4 function correlates with poorly differentiated carcinoma, with aggresive phenotype and a highly lethal outcome.  
Product Image Gallery
Western blot analysis of extracts on K562, Jurkat, HEK293 cells, using Brd4 Mouse Monoclonal Antibody (EAB22485) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Predicted band size: 152 kDa; Observed band size: 200 kDa
Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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