SKU-Pack Size | Availability | Size | Price | |
EAB22485-100UL | In Stock | 100ul | €336.70 |
Please select the country you are in to find your local distributor. |
OlaSciences | Phone£º+33 (0) 9 77 4001 556 | |
87, rue des Suisses 65000 Nanterre, | E-mail£ºola@olasciences.com | |
France | France | Web£ºwww.olasciences.com |
Product Information | |
Applications | WB, IP, ChIP |
Species Reactivity | Human, Mouse |
Host / Isotype | Mouse IgG1 |
Clonality | Monoclonal |
Applications Dilutions | WB=1:1000-5000¡¡¡¡ ChIP=1:100-500 |
MW (kDa) | 152 |
Conjugate | Unconjugate |
Specificity | Brd4 Mouse Monoclonal Antibody detects endogenous levels of Brd4 protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20¡ãC least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4¡ãC for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
Product Bioinformatics | |
Synonym(s) | Bromodomain containing 4; CAP; MCAP; CDLS6; HUNK1; HUNKI; FSHRG4; BRD4 |
Gene Aliases | BRD4 |
UniProt ID | |
Entrez Gene ID |
Product Description | |
Brd4 belongs to the BET family, a group of structurally related proteins containing two bromodomains. Through these two domains, Brd4 associates with mitotic chromosomes and its expression correlates with cell growth. Expression of Brd4 inhibits cell cycle progression from G1 to S, due to binding to the largest subunit of replication factor C (RFC) to prevent DNA elongation. Altered Brd4 function correlates with poorly differentiated carcinoma, with aggresive phenotype and a highly lethal outcome. |
Product Image Gallery | |
Western blot analysis of extracts on K562, Jurkat, HEK293 cells, using Brd4 Mouse Monoclonal Antibody (EAB22485) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 152 kDa; Observed band size: 200 kDa |
Specific Protocols | |