Polyclonal Antibodies - Brd4 Rabbit Polyclonal Antibody

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Brd4 Rabbit Polyclonal Antibody

Catalog #：EAB11446

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB11446-30UL	In Stock	30ul	€115.70
EAB11446-100UL	In Stock	100ul	€258.70
EAB11446-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	OlaSciences	Phone：+33 (0) 9 77 4001 556
	87, rue des Suisses 65000 Nanterre,	E-mail：ola@olasciences.com
France	France	Web：www.olasciences.com

Product Information
Applications	WB, IP
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IP=1:20-200
MW (kDa)	152
Conjugate	Unconjugate
Specificity	Brd4 Rabbit Polyclonal Antibody detects endogenous levels of Brd4 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Bromodomain containing 4; CAP; MCAP; CDLS6; HUNK1; HUNKI; FSHRG4; BRD4
Gene Aliases	BRD4
UniProt ID	O60885
Entrez Gene ID	23476

Product Description

Brd4 belongs to the BET family, a group of structurally related proteins containing two bromodomains. Through these two domains, Brd4 associates with mitotic chromosomes and its expression correlates with cell growth. Expression of Brd4 inhibits cell cycle progression from G1 to S, due to binding to the largest subunit of replication factor C (RFC) to prevent DNA elongation. Altered Brd4 function correlates with poorly differentiated carcinoma, with aggresive phenotype and a highly lethal outcome.

Product Image Gallery
Western blot analysis of extracts on 293, K562 cells, using Brd4 Rabbit Polyclonal Antibody (EAB11446) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol