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Twinfilin-1 Mouse Monoclonal Antibody
Catalog #:EAB22401
  SKU-Pack Size Availability Size Price
EAB22401-100UL In Stock 100ul $343.50
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Product Information
Applications WB, IHC-P, IF/ICC, FC, ELISA
Species Reactivity Human
Host / Isotype Mouse IgG1
Clonality Monoclonal
Applications Dilutions WB=1:500-2000 
IHC-P=1:100-500 
IF/ICC=1:50-300
FC=1:50-200
MW (kDa) 40
Conjugate Unconjugate
Specificity Twinfilin-1 Mouse Monoclonal Antibody detects endogenous levels of Twinfilin-1 protein.
Purification Affinity purification
Concentration 1mg/ml
Format Liquid
Formulation In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping Gel Pack
Storage Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin
IF
-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry

Reactivity Key

H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish
Hr
-Horse All-All Species Expected

Product Bioinformatics
Synonym(s) Twinfilin actin binding protein 1; A6; PTK9; TWF1
Gene Aliases TWF1
UniProt ID

Q12792

Entrez Gene ID

5756

Product Description

Twinfilin is a highly conserved Actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. Twinfilin is composed of two ADF-homology domains; it coordinates filament severing and monomer sequestering at sites of rapid Actin turnover, thus preventing assembly of the monomer into filaments. Twinfilin-1 is the mammalian homolog and is expressed in embryos and in most adult non-muscle cell types. Twinfilin-1 binds ADP-G-Actin and efficiently halts Actin filament assembly by inhibiting the nucleotide exchange on Actin monomers and directly interacting with the capping protein.  

Product Image Gallery
Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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