Monoclonal Antibodies - Twinfilin-1 Mouse Monoclonal Antibody

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Twinfilin-1 Mouse Monoclonal Antibody

Catalog #：EAB22401

	SKU-Pack Size	Availability	Size	Price
	EAB22401-100UL	In Stock	100ul	€297.70

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email us at info@ebiocell.com

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	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IHC-P, IF/ICC, FC, ELISA
Species Reactivity	Human
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:50-300 FC=1:50-200
MW (kDa)	40
Conjugate	Unconjugate
Specificity	Twinfilin-1 Mouse Monoclonal Antibody detects endogenous levels of Twinfilin-1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Twinfilin actin binding protein 1; A6; PTK9; TWF1
Gene Aliases	TWF1
UniProt ID	Q12792
Entrez Gene ID	5756

Product Description

Twinfilin is a highly conserved Actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. Twinfilin is composed of two ADF-homology domains; it coordinates filament severing and monomer sequestering at sites of rapid Actin turnover, thus preventing assembly of the monomer into filaments. Twinfilin-1 is the mammalian homolog and is expressed in embryos and in most adult non-muscle cell types. Twinfilin-1 binds ADP-G-Actin and efficiently halts Actin filament assembly by inhibiting the nucleotide exchange on Actin monomers and directly interacting with the capping protein.

Product Image Gallery

Western blot analysis of extracts on HEK293 cells, using Twinfilin-1 Mouse Monoclonal Antibody (EAB22401) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue, using Twinfilin-1 Mouse Monoclonal Antibody (EAB22401) at 1:200 dilution.

Immunofluorescence analysis of Hela cell, using Twinfilin-1 Mouse Monoclonal Antibody (EAB22401)(green) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol