Q12792

Twinfilin is a highly conserved Actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. Twinfilin is composed of two ADF-homology domains; it coordinates filament severing and monomer sequestering at sites of rapid Actin turnover, thus preventing assembly of the monomer into filaments. Twinfilin-1 is the mammalian homolog and is expressed in embryos and in most adult non-muscle cell types. Twinfilin-1 binds ADP-G-Actin and efficiently halts Actin filament assembly by inhibiting the nucleotide exchange on Actin monomers and directly interacting with the capping protein.