Monoclonal Antibodies - MIST1 Mouse Monoclonal Antibody

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MIST1 Mouse Monoclonal Antibody

Catalog #：EAB22493

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22493-100UL	In Stock	100ul	€336.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB
Species Reactivity	Human, Mouse, Rat, Pig
Host / Isotype	Mouse IgG2a
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000
MW (kDa)	21
Conjugate	Unconjugate
Specificity	MIST1 Mouse Monoclonal Antibody detects endogenous levels of MIST1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Basic helix-loop-helix family member a15; MIST1; BHLHB8; BHLHA15
Gene Aliases	BHLHA15
UniProt ID	Q7RTS1
Entrez Gene ID	168620

Product Description

MIST1 also known as BHLHB8, BHLHA15 is a 189 amino acid nuclear protein expressed in liver, brain, skeletal muscle and spleen. MIST1 contains a basic helix-loop-helix (bHLH) domain and belongs to the bHLH family of transcription factors. Members of this family bind to the E-box motifs present in the promoter or enhancer regions of a variety of developmentally regulated genes and function as either transcriptional activators or transcriptional repressors. MIST1 is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins (E12 and E47) and is believed to play a role regulating the transcriptional activity of MyoD, a protein involved in the regulation of muscle cell development. More specifically, MIST1 functions as a repressor of MyoD activity, ensuring that myoblast populations do not differentiate. In addition, MIST1 is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development.

Product Image Gallery
Western blot analysis of extracts on different cells, using MIST1 Mouse Monoclonal Antibody (EAB22493) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Lane1: BxPC3 Lane2: Rat pancreas

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol