Monoclonal Antibodies - CHD4 Mouse Monoclonal Antibody

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CHD4 Mouse Monoclonal Antibody

Catalog #：EAB21169

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21169-100UL	In Stock	100ul	$343.50

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email us at info@ebiocell.com

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
Canada	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IP, IF/ICC
Species Reactivity	Human
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IP=1:50-200　 IF/ICC=1:50-300
MW (kDa)	218
Conjugate	Unconjugate
Specificity	CHD4 Mouse Monoclonal Antibody detects endogenous levels of CHD4 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Chromodomain helicase DNA binding protein 4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA; CHD4
Gene Aliases	CHD4
UniProt ID	Q14839
Entrez Gene ID	1108

Product Description

CHD4 also known as Mi-2b, Mi2-BETA belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Image Gallery
Western blot analysis of extracts on different cells, using CHD4 Mouse Monoclonal Antibody (EAB21169) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Lane1: Hela Lane2: U2OS Predicted band size: 218 kDa; Observed band size: 260 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol