SKU-Pack Size | Availability | Size | Price | |
EAB22711-100UL | In Stock | 100ul | £284.90 |
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CUlabSciences | Phone:+44 (0) 1232 330008 | |
Cambridge House St Thomas' Place Ely, | E-mail:sales@culabsciences.co.uk | |
United Kingdom | Cambridge CB27 9RD UK | Web:www.culabsciences.co.uk |
Product Information | |
Applications | WB, IP, IHC-P |
Species Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse IgG2b |
Clonality | Monoclonal |
Applications Dilutions | WB=1:5000-20000 IP=1:100-500 IF/ICC=1:500-2000 |
MW (kDa) | 20 |
Conjugate | Unconjugate |
Specificity | CRYAB Mouse Monoclonal Antibody detects endogenous levels of CRYAB protein. |
Purification | Affinity purification |
Concentration | 1mg/ml |
Format | Liquid |
Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
Shipping | Gel Pack |
Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
Research Use | For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. |
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
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Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
Product Bioinformatics | |
Synonym(s) | Crystallin alpha B; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101; CRYAB |
Gene Aliases | CRYAB |
UniProt ID | |
Entrez Gene ID |
Product Description | |
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. |
Product Image Gallery | |
Western blot analysis of extracts on different cells, using CRYAB Mouse Monoclonal Antibody (EAB22711) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: Mouse eye Lane2: Rat eye |
Specific Protocols | |