Monoclonal Antibodies - NSE Mouse Monoclonal Antibody

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NSE Mouse Monoclonal Antibody

Catalog #：EAB11299

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB11299-30UL	In Stock	30ul	￡97.90
EAB11299-100UL	In Stock	100ul	￡218.90
EAB11299-200UL	In Stock	200ul	￡372.90

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IHC-P=1:100-500　 IF/ICC=1:100-500
MW (kDa)	47
Conjugate	Unconjugate
Specificity	NSE Mouse Monoclonal Antibody detects endogenous levels of NSE protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Enolase 2; NSE; HEL-S-279; ENO2
Gene Aliases	ENO2
UniProt ID	P09104
Entrez Gene ID	2026

Product Description

NSE also known as γ Enolase, ENO2 is one of three isoenzymes of enolase, which are highly conserved cytoplasmic glycolytic enzymes crucial for energy metabolism. NSE plays a crucial role in the glycolytic pathway, specifically in the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is a key step in energy production. NSE is predominantly expressed in the nervous system, making gamma Enolase (NSE-P1) antibody an important marker for neuronal differentiation and function. NSE presence in neural tissues, as opposed to α Enolase and β Enolase isoforms found in various tissues and muscle respectively, highlights NSE′s significance in neurological studies and potential implications in neurodegenerative diseases.

Product Image Gallery

Western blot analysis of extracts on Hela, Jurkat, 293T cells, using NSE Mouse Monoclonal Antibody (EAB11299) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, using NSE Mouse Monoclonal Antibody (EAB11299) at 1:200 dilution.

Immunofluorescence analysis of Hela cell, using NSE Mouse Monoclonal Antibody (EAB11299)(green) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol