Monoclonal Antibodies - CHD3 Mouse Monoclonal Antibody

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CHD3 Mouse Monoclonal Antibody

Catalog #：EAB21527

	SKU-Pack Size	Availability	Size	Price
	EAB21527-100UL	In Stock	100ul	€258.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IHC-P, IF/ICC, FC
Species Reactivity	Human, Mouse
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:100-500　 FC=1:50-200
MW (kDa)	226
Conjugate	Unconjugate
Specificity	CHD3 Mouse Monoclonal Antibody detects endogenous levels of CHD3 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Chromodomain helicase DNA binding protein 3; ZFH; Mi-2a; SNIBCPS; Mi2-ALPHA; CHD3
Gene Aliases	CHD3
UniProt ID	Q12873
Entrez Gene ID	1107

Product Description

CHD3 also designated Mi2-α is a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

Product Image Gallery

Western blot analysis of extracts on different cells, using CHD3 Mouse Monoclonal Antibody (EAB21527) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: Hela
Lane2: Jurkat
Lane3: HEK293
Lane4: K562
Lane5: Mouse brain

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue, using CHD3 Mouse Monoclonal Antibody (EAB21527) at 1:200 dilution.

Immunofluorescence analysis of Hela cell, using CHD3 Mouse Monoclonal Antibody (EAB21527) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol