Enolase-1 also known as α Enolase, ENO1 is a member of the enolase family. Enolase-1 plays a crucial role in the glycolytic pathway, facilitating the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is a key step in energy production. This enzymatic function is vital for cellular metabolism, particularly in tissues with high energy demands, such as muscle and brain. Notably, Enolase-1 is not only involved in glycolysis but also has been implicated in various cellular processes, including differentiation and stress responses. Enolase-1 expression across a wide range of tissues underscores its importance in maintaining metabolic homeostasis, and expression levels can serve as a biomarker for certain pathological conditions. With an ability to form both homodimers and heterodimers with other enolase isoforms, Enolase-1′s structural versatility further enhances its functional significance in cellular metabolism.