Monoclonal Antibodies - IBA1 Mouse Monoclonal Antibody

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IBA1 Mouse Monoclonal Antibody

Catalog #：EAB22150

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22150-100UL	In Stock	100ul	$343.50

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
Canada	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:100-500
MW (kDa)	16
Conjugate	Unconjugate
Specificity	IBA1 Mouse Monoclonal Antibody detects endogenous levels of IBA1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	IBA1; IRT1; AIF-1; IRT-1; AIF1; Allograft inflammatory factor 1
Gene Aliases	AIF1
UniProt ID	P55008
Entrez Gene ID	199

Product Description

IBA1 also known as allograft inflammatory factor-1 (AIF-1) is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

Product Image Gallery

Western blot analysis of extracts on different cells, using IBA1 Mouse Monoclonal Antibody (EAB22150) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Lane1: THP-1
Lane2: Mouse liver
Lane3: Rat brain

Immunohistochemical analysis of paraffin-embedded Human brain tissue, using IBA1 Mouse Monoclonal Antibody (EAB22150) at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue, using IBA1 Mouse Monoclonal Antibody (EAB22150) at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue, using IBA1 Mouse Monoclonal Antibody (EAB22150) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol