Polyclonal Antibodies - Phospho-SPAK (Ser309) Rabbit Polyclonal Antibody

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Phospho-SPAK (Ser309) Rabbit Polyclonal Antibody

Catalog #：EAB11145

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB11145-30UL	In Stock	30ul	€141.70
EAB11145-100UL	In Stock	100ul	€297.70
EAB11145-200UL	In Stock	200ul	€479.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IHC-P, IF, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF=1:50-300
MW (kDa)	59
Conjugate	Unconjugate
Specificity	Phospho-SPAK (Ser309) Rabbit Polyclonal Antibody detects endogenous levels of SPAK protein when phosphorylated at Ser309.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Serine threonine kinase 39; DCHT; PASK; SPAK
Gene Aliases	STK39
UniProt ID	Q9UEW8
Entrez Gene ID	27347

Product Description

SPAK, also known as STK39 (serine threonine kinase 39), DCHT or PASK, is a 547 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one protein kinase domain. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Product Image Gallery
Western blot analysis of extracts on COLO205 cells, using Phospho-SPAK (Ser309) Rabbit Polyclonal Antibody (EAB11145) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol